Table 3.
Transmission study of human, genetic mutant Q227X PrP to tg66 transgenic mice expressing human PrP
| Mouse number | DPI | PrPSc IHC | PrPSc western blot |
Clinical TSE suspect | Clinical notes (reason for euth) & relevant necropsy findings |
|---|---|---|---|---|---|
| B302–2 | 77a | – | nt | No | normal |
| B299–1 | 77a | – | nt | No | normal |
| B302–3 | 239a | – | nt | No | normal |
| B302–4 | 239a | – | nt | No | normal |
| B304–1 | 529a | – | nt | No | normal |
| B304–2 | 529a | – | nt | No | normal |
| B630–1 | 545 | – | – | No | lung neoplasia |
| B296–1 | 650 | – | – | No | eye neoplasia |
| B295–1 | 696 | – | – | No | injured, thin, ataxic, adequate nesting, lymphoma |
| B296–2 | 716 | – | – | No | injured, thin, barrel rolled twice, aware and responsive |
| B295–2 | 743 | – | – | No | distended abdomen, liver neoplasia |
| B296–3 | 756 | – | – | No | thin |
| B306–3 | 782a | – | nt | No | normal |
| B306–4 | 782a | – | nt | No | normal |
| B340–1 | 784 | – | – | No | urine scalding |
| B295–3 | 798 | – | – | No | normal |
a- Indicates mice that were stereotactically microinjected into the striatum. Mice injected using this technique were euthanized electively and tissues were processed to directly screen the injection needle track and adjacent brain by IHC for any PrP replication. Mice without asterisks were intracerebrally inoculated with a 30ul volume of brain homogenate and euthanized when they developed neurologic signs consistent with prion infection (none) or conditions requiring euthanasia for humane reasons or at the termination of the experiment at 782 and 798 dpi