Table 4.
Transmission study of human, genetic mutant G131V PrP to tg66 transgenic mice expressing human PrP
| Mouse number | DPI | PrPSc IHC | PrPSc western blot | Clinical TSE suspect | Clinical notes (reason for euth) & relevant necropsy findings |
|---|---|---|---|---|---|
| B300–3 | 79a | – | nt | No | normal |
| B339–3 | 79a | – | nt | No | normal |
| B307–1 | 241a | – | nt | No | normal |
| B307–2 | 241a | – | nt | No | normal |
| B308–3 | 531a | + | nt | No | normal |
| B328–1 | 531a | + | nt | No | normal |
| B633–1 | 596 | + | – | No | normal |
| B297–1 | 650 | + | – | No | weight loss, hind limb weakness |
| B297–2 | 731 | + | – | Yes | head bob, tremors, thin |
| B298–1 | 731 | + | – | Yes | weak hind legs, thin, kyphosis, weepy eye, ataxia for 1 month |
| B297–3 | 735 | + | – | Yes | thin, slight wobble, rough coat, squinty eyes, decent nest |
| B297–4 | 735 | + | – | Yes | thin, slight wobble, rough coat, squinty eyes, decent nest |
| B632–1 | 758 | + | – | Yes | thin, rough coat, mild ataxia |
| B298–2 | 769 | + | – | Yes | thin, ataxic |
| B303–1 | 784 | + | – | Yes | urine scalding, weak hind legs, tremor |
a- Indicates mice that were stereotactically microinjected into the striatum. Mice injected using this technique were euthanized electively and tissues were processed to directly screen the injection needle track and adjacent brain by IHC for any PrP replication. Mice without asterisks were intracerebrally inoculated with a 30ul volume of brain homogenate and euthanized when they developed neurologic signs consistent with prion infection or conditions requiring euthanasia for humane reasons