Fig. 2.

Overexpression of CLCA2 inhibits the growth in NPC cells in vitro and in vivo. a Overexpression of CLCA2 in NPC cells were determined by real-time quantitative PCR, normalized to β-actin. b Overexpression of CLCA2 in NPC cells were determined by immunoblotting analysis, GAPDH was used as a loading control. c Overexpression of CLCA2 inhibits cells proliferation was determined by the MTS assay; **P < 0.01, result of Student t test. d-e Overexpression of CLCA2 decreased cell colony numbers; representative micrographs and quantification of crystal violet stained cells from 3 independent experiments. f Overexpression of CLCA2 in 5-8F cells and the vector control cells were subcutaneously injected into nude mice. g The terminal tumor weights are decreased compared with the control group, *P < 0.05, **P < 0.01, result of Student t test. h The growth curve indicates 5-8F growth suppression upon CLCA2 overexpression in vivo, the results are presented as the mean ± SD, n = 6 per group, scale bar, 1 cm