Fig. 6.

Functional analysis of CRISPR/Cas9-directed mutation correction of a β-catenin Ser45 mutation in HCT-116 cells. a depicts the CRISPR/Cas9 targeted mutation site (ΔTCT Ser45) in green letters and surrounding sequences in exon 3 of β-catenin gene. Multiple serine and threonine genetic codes around the target site are marked in red-color letters. b illustrates the disruption rate induced by transfecting the Cas9-eGFP-β-catenin-gRNA (ΔTCT Ser45) co-expression vector in HCT-116 cells. c shows a representative Western blot analysis of β-catenin Ser45 phosphorylation [p-β-catenin (ser45)] and total β-catenin expression in the parental and multiple mutation-corrected HCT-116 cell clones. β-actin was used as an internal control. d shows a representative image of colony formation assay for the parental control and a mutation-corrected HCT-116 cell clone. e is a quantification of colony formation in parental control and mutation-corrected HCT-116 cells. Colony formation is presented as percentage normalized to the parental control. The data is the mean ± SD of 4 independent experiments. *p < 0.05 compared to the parental control