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. 2018 Feb 20;17:27. doi: 10.1186/s12934-018-0870-6

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Electrophoresis results of the construction of transgenic strains on a 1% agarose gel. A PCR products of target gene; B purified target gene from PCR products; C lane a: ppk+pEASY-Blunt vector; line b: ppk+pEASY-Blunt vector digested with HindIII and KpnI restriction enzymes; line c: pSyn_1 vector; line d: pSyn_1 vector digested with HindIII and KpnI restriction enzymes; D lane a: ppk+pSyn_1 vector; line b: ppk+pSyn_1 vector digested with HindIII or KpnI restriction enzymes; line c: ppk+pSyn_1 vector digested with HindIII and KpnI restriction enzymes; E PCR products from ppk-type strain plasmid (lane a and b); M in A, B, E was 5 Kb DNA Marker, in C, D was 1 Kb DNA Ladder