Fig. 6.

In vitro effect of estrogen on tumorigenesis properties of ETV4-knockdown CCA cell lines. Spheroid proliferation assays were performed as triplicated experiments and in vitro invasion experiments were performed as duplicated experiments. a and b Estrogen-stimulated spheroid growth of scramble- and ETV4-knockdown. a KKU-213 and b KKU-139 CCA cell lines in a 3D system. Arrows indicate the variables compared for statistical significance. c and d Representative pictures of spheroids of scramble- and ETV4-knockdown of c KKU-213 and d KKU-139. e In vitro invasion assay of scramble- and ETV4- knockdown of KKU-213 and KKU-139 CCA cells stimulated by E2. Symbol * determined statistically significant difference compared to scramble without E2 treatment with P < 0.05