Fig. 2.

Cholesterol depletion induces programmed cell death. a MDA-MB 231 cells were treated with 5 mMMβCD for 2, 4 and 8 h. Cells were stained with Annexin V and PI. Exhibition of phosphatidylserine (PS) and cell death was measured by flow cytometer. b Phosphatidylserine exhibition by MDA-MB 231 and MCF-7 Cell line after 5 mM MβCD exposure for 2 and 4 h. c, d Flow cytometer and fluorescence microscopy analysis of membrane potential assay by JC-1 staining after 4 h exposure with 5 mM MβCD. Statistical analysis: One way anova, post hock test Dunnett’s test, *P < 0.05, **P < 0.01, **P < 0.001, N.S. not significant