Fig. 4.

Assessment of role of necroptosis and pyroptosis. MDA-MB 231 cells were incubated with 5 mM MβCD in the presence and absence of necrostatin-1 (40 µg/ml) (a), Z-VAD [OME]-FMK (20 µg/ml) and necrostain-1 (40 µg/ml) (b) and 5 mM glycine along with Z-VAD [OME]-FMK (20 µg/ml (c) for 6 h. Cell proliferation was measured by MTT. Statistical analysis: One way anova, post hock test Tukey. *P < 0.05, **P < 0.01, **P < 0.001, N.S. not significant