Fig. 5.

Nilotinib activates ERK1/2 and AKT pathways in myoblasts. Representative Western blot analyses from three independent experiments to evaluate the phosphorylation of (a) p44/42 MAPK (ERK1/2) (Thr202/Tyr204) and (b) AKT (S473) in myoblasts after Nilotinib treatment at different concentrations. Total ERK1/2 and AKT were used as loading controls. c Representative Western blots analysis from three independent experiments that evaluate the phosphorylation of ERK1/2 and AKT in myoblasts after Nilotinib treatment at different time points. Total ERK1/2 and AKT were used as loading controls. d Representative Western blots analysis from three independent experiments that evaluate the phosphorylation of ERK1/2 and AKT in 7 days myotubes. Ara-C non-treated and Ara-C-treated myotubes are shown. Nilotinib treatment was performed for 1 h. Total ERK1/2 and AKT were used as loading controls