| Gene | Forward primer | Reverse primer |
| MyoD | 5’–GCCGCCTGAGCAAAG | 5’–CAGCGGTCCAGTGCG |
| TGAATG–3’ | TAGAAG–3’ | |
| Myogenin | 5’–GTCCCAACCCAGGAG | 5’–CCACGATGGACGTAA |
| ATCAT–3’ | GGGAG–3’ | |
| CyclinD1 (Ccnd1) | 5’-CCCAACAACTTCCTC | 5’-TCCAGAAGGGCTTCA |
| TCCTG−3’ | ATCTG−3’ | |
| 18S | 5’–TGACGGAAGGGCA | 5’–CACCACCACCCA |
| CCACCAG–3’ | CGGAATCG–3’ |
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