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. 2018 Feb 20;18:69. doi: 10.1186/s12906-018-2117-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Histological and dynamic histological analysis of articular cartilage and subchondral bone in experimental groups. a 3D μ-CT images of articular cartilage. b safranin-O staining (c) toluidine blue staining of sagittal sections of tibia. Double headed arrow showing hyaline cartilage and calcified cartilage (d) OARSI score from five different sections n = 3 per group. Micro-CT analysis of sub-chondral bone (e) total tissue volume (TV), (f) bone volume/tissue volume (BV/TV and (g) trabecular porosity (Tb. Pf). All values are expressed as Mean ± S.E.M. (n = 6/group); *P < 0.05, **P < 0.01, ***P < 0.001 compared to the MIA group ^#P < 0.05, ^##P < 0.01, compared to the 250 mg.kg^− 1 day^− 1 dose, NS= non significant