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. Author manuscript; available in PMC: 2019 Feb 15.
Published in final edited form as: Mol Cell. 2018 Feb 15;69(4):610–621.e5. doi: 10.1016/j.molcel.2018.01.024

Figure 4. Restoration of one-carbon units to the mitochondria is required to rescue mitochondrial defects observed upon SHMT2 deletion.

Figure 4

A, C, E, Immunoblot from cell lysates of Jurkat cell lines for proteins encoded by the indicated genes, upon addition of the indicated concentration of sodium formate for 3 days. B, Proliferation of Jurkat cells or clones from A, treated with the indicated concentration of sodium formate for 3 days prior to measurement. Cells were then grown for 5 days in media initially containing 10 mM (high glucose) or 1.5 mM glucose (low glucose). D, Basal oxygen consumption rate (OCR) of the cell lines from C, cultured in 10 mM glucose and the indicated concentration of sodium formate for 3 days. F, Above, proliferation of Jurkat cells or clones expressing the indicated sgRNAs and cDNAs. Below, data from above, normalized to the 10 mM glucose condition for each cell line. Cells were grown for 5 days in media initially containing 10 mM (high glucose) or 1.5 mM glucose (low glucose). * p < 0.05, ns = not significant. Error bars are s.e.m. n=3.