Figure 8.
A schematic of the integrated SELEX chip by Huang et al. (A) The bottom glass layer is patterned with microheaters and sensors (platinum) with associated electrodes (gold) for temperature control during PCR. The thin-film PDMS membrane and thick PDMS block facilitate all liquid handling, including pumping and mixing by pneumatic membrane deflection. (B) The assembled device contains a main incubation/PCR chamber connected to chambers for wash buffer, PCR reagents, and waste by low-volume microchannels to prevent dead space and bubble formation. Adapted from Huang et al93 with permission from Elsevier.