Fig. 1.

Methyltransfer reactions in gentamicin biosynthetic pathway. The determined major and minor alternative pathways in the methylation network are indicated by boldface type and solid arrows, respectively. The dashed arrows refer to conversions without substantial experimental support. Methyltransferases and their roles revealed in this study of GenN, GenD1, GenK, and GenL are highlighted in magenta, orange, blue, and red, respectively. The extents of methylation are shown in the dashed rectangle boxes. Carbon numbering is shown with the structure of 1.