Fig. 1.
Myeloid cell egress from the bone marrow and homing to lymphoid organs during EAE are compromised in the absence of IL-1β. (A–D) Quantification of the number of neutrophils, CD4+ T cells, as well as Ly6Chi and Ly6Clow monocytes (CD115+) in the bone marrow (A and B) and blood (C and D) of unimmunized (naive) mice and immunized (EAE) mice killed at 7 d.p.i. (E and F) Quantification of the number of neutrophils, CD4+ T cells, Ly6Chi monocytes/macrophages (Ly6Chi Mo/MØ), moDCs, and DCs present in the spleen of WT and Il1b−/− mice at 7 d.p.i. Data are shown either as a percentage of CD45 leukocytes (A, C, and E) or as total number of cells per femur (B), milliliter of blood (D), or spleen (F). *P < 0.05, **P < 0.01, ***P < 0.001; two-way ANOVA followed by a Bonferroni post hoc test; data shown are mean ± SEM, n = 5–6 animals per group. Data are representative of at least two independent experiments.