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. 2018 Jan 23;115(6):1388–1393. doi: 10.1073/pnas.1715556115

Fig. 1.

Fig. 1.

Plasma membrane/plasmodesmal C4 suppresses cell-to-cell spread of RNAi. (A) Subcellular localization of C4-GFP or the nonmyristoylable mutant C4G2A-GFP upon transient expression in Nicotiana benthamiana leaves. BF, Bright field; AF, Autofluorescence. Empty arrowheads indicate plasmodesmata; filled arrowheads indicate chloroplasts. (Scale bar, 25 µm.) (B) Transgenic plants expressing C4 or the nonmyristoylable mutant C4G2A at 5 wk postgermination. (Scale bar, 0.5 cm.) (C) Leaves of 4-wk-old transgenic SUC:SUL plants expressing C4 (S-S/35S:C4), the nonmyristoylable mutant C4G2A (S-S/35S:C4G2A), or transformed with the empty vector (S-S/EV). Each set of four leaves comes from one T2 plant from an independent line. (Scale bar, 0.5 cm.) (D) Quantification of the bleaching percentage of the leaves in B. Error bars represent SE; bars with the same letter are not significantly different (P = 0.05) according to Dunnet’s multiple comparison test.