Fig. 5.

Role of archaeal adaptor protein CheF. Effect of CheF on directed movement in H. volcanii H26 in rich medium. Different plasmids were expressed in the ΔcheF background, strains spotted on semisolid agar plates, and plates scanned after 5 d. (A) Representative example of motility assay on semisolid agar plates of different H. volcanii strains. (B) Average diameter of motility rings, measured relative to the WT, from different H. volcanii strains from more than three independent experiments including three biological replicates each. pTA1228 is the empty plasmid. Error bar indicates SD. (C) Analysis of swimming behavior of different H. volcanii mutants as observed with time-lapse movies on the thermomicroscope. Tukey box plot of number of seconds between two subsequent turns of >90° of a cell. For each strain, the median is displayed above the respective bar and indicated by a black horizontal line. Boxes display the 25–75th percentile and bars represent the minimum and maximum time. Circle indicates maximum outliers. Asterisk indicates significantly different swimming behavior from WT (P < 0.005) as established by t test (SI Appendix, Table S2). (Magnification: A, 0.7×.)