Table 1.

FWHM of the PSF measured in cryoconditions ($-140°$C) and at room temperature by using the prototyped cryoimmersion objective ($63\times /1.15$) and comparison with an air objective ($63\times /0.75$)

Observation conditions	${\text{FWHM}}_{xy}$ (nm)	${\text{FWHM}}_z$ (nm)	Peak intensity (a.u.)
Cryoimmersion objective W2010 @ 23 °C	295 $\pm$ 6	902 $\pm$ 17	8,444 $\pm$ 484
Cryoimmersion objective HFE 7200 @ −140 °C	353 $\pm$ 16	1,554 $\pm$ 58	3,449 $\pm$ 362
Air objective 0.75 NA @ −140 °C	385 $\pm$ 14	1,743 $\pm$ 108	610 $\pm$ 165