Fig. 3.

Adipose tissue morphology and mRNA levels in chow-fed A3^−/−A8^−/− and WT littermates. (A) Representative images of tissue chunks (Upper) and H&E staining of adipose tissue sections (Lower) (n = 4; age 18 wk; three fields per mouse). (Magnification: 20×.) The experiment was repeated twice, and the results were similar. (Scale bars: 50 μm.) (B) Fold changes in levels of selected mRNAs encoding proteins involved in fatty synthesis and oxidation (Upper) and glycolysis and mitochondrial function (Lower) in WAT-SQ of male WT and A3^−/−A8^−/− mice (n = 3 per group; age 18 wk) fasted overnight and then fed for 4 h. Transcript levels were determined by RNA-seq as described in Materials and Methods. Values are means ± SEM. Groups were compared using unpaired t tests. *P < 0.05; **P < 0.01; ***P < 0.001. The experiment was repeated once, and the results were similar.