Fig. 4.

Mitochondrial number, protein content, and size in WAT-SQ from A3^−/−A8^−/− and WT mice. (A) Ratio of mitochondrial to nuclear DNA (Left) and mitochondrial protein content (Right) of WAT-Epi, WAT-SQ, and BAT was determined by using mitochondria isolated by centrifugation. The amount of protein was normalized by tissue sample weight (n = 6 mice per group; age 11–13 wk). (B) Electron micrographs (Left) and mean (±SE) diameter (Right) of the mitochondria from WAT-SQ. (Scale bars: 0.5 μm.) (C and D) A Seahorse XF24 analyzer was used to measure extracellular OCRs in adipose tissue in mice fed a chow diet ad libitum (C) and after a 14-h fast (D) (n = 6; with five replicates per mouse). Values are means ± SEM. Groups were compared by using unpaired t tests. *P < 0.05; ****P < 0.00001.