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. 2018 Jan 16;115(6):E1137–E1146. doi: 10.1073/pnas.1717910115

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Fig. 6. — Translation initiation factor eIF4E1-enhanced translation promotes cytocapsular tube development. (A) Western blotting assays of HMECs and BCSCs during cytocapsular tube development. (B and C) Quantitation of cytocapsula generation (n = 3) (B) and cytocapsular tube elongation (n = 3) (C). (D) Immunohistochemistry assays of cytocapsular tubes in BCSC tumors with compound treatments. The cytocapsular tube (CT; white arrows) and cytocapsular tube membrane (CTM; orange arrow) are shown. (Scale bars: 10 µm.) (E) Quantitation of cytocapsular tubes in D (n = 5 specimens from five mice of each group, one specimen per mouse). The cytocapsular tube numbers in the mice in the control group was set as 100%, and the relative cytocapsular tube quantitation is shown. (F) Western blotting assays in D. (G) Quantitation of gene transcription in D; ns, P > 0.05; ***P < 0.001.