Fig. 6.

AHSP and Hb-α have opposite impacts on Hb-β expression in nonerythroid cells. HEK cells transiently expressing Myc-tagged globins either individually or in combination with AHSP were analyzed for protein expression and protein associations. (A) Expression levels of Myc-tagged globins, AHSP, and loading control β-actin in HEK cells under conditions as indicated. (Right) Western blots probed with specific Hb antibodies. (B) Corresponding densitometry of protein expression levels. Values depicted are mean ± SD of three independent experiments (n = 3) (*P < 0.05, by one-way ANOVA). (C) IPs showing bound hsp90 and AHSP associated with Myc-Hb (input 10%) retained on the beads. (Lower three panels) The corresponding expression levels of individual globins and β-actin as indicated.