Fig. 1.
Effects of temperature on cofactor binding affinity (KMNADH) (Left) and time-dependent loss of activity (Right) for 12 orthologs of cMDH from five genera of marine molluscs (mean ± SEM, n = 5, genus Echinolittorina; mean ± SEM, n = 3, genus Nerita; mean ± SEM, n = 3, genus Lottia; mean ± SEM, n = 3, genus Littorina; mean ± SEM, n = 3, genus Chlorostoma). Approximate upper lethal temperatures for the species are E. malaccana (55 °C), Echinolittorina radiata (52 °C), Nerita albicilla (50 °C), Nerita yoldii (50 °C), L. austrodigitalis (41 °C), L. digitalis (40 °C), Littorina keenae (48 °C), Littorina scutulata (45 °C), C. rugosa (∼45 °C), C. funebralis (42 °C), C. brunnea (36 °C), and C. montereyi (36 °C). (See Fig. S1 for biogeographic information and sources of thermal tolerance data. Slopes of rates of loss of activity are given in Table S1).