Fig. 3.

MDS analysis of side chain movements (RMSF) in molluscan cMDHs. RMSF spectra for congeners of Echinolittorina (A) and Chlorostoma (B) at simulation temperatures of 15 and 42 °C (n = 3). Values for average RMSF across the full sequence are given in Table S1. (C and D) Number of residues showing a change in RMSF (ΔRMSF) > 0.5 Å vs. the species’ lethal temperature (LT₅₀) (C) and the rate of thermal denaturation (D) for the cMDH orthologs. (E and F) Number of residues occurring in MR regions (residues 90–105 and 230–245) showing ΔRMSF > 0.5 Å vs. LT₅₀ (E) and the rate of thermal denaturation (F) for the cMDH orthologs. The numbers assigned to each species are given in the Fig. 2 legend. The relationships between the number of residues showing significant change and the LT₅₀ or rate of thermal denaturation were analyzed by least-squares linear regression.