Table 1.

Conserved signature indels (CSIs) that are specific for different members of the genus Mycobacterium and those which are lacking only in members of the “Abscessus-Chelonae” clade.

Protein name	Accession number	Figure number	Indel size	Indel position	Specificity
Ergothioneine biosynthesis protein EgtBa	WP_062878530	Figure 3 Supplementary Figure 4	2aa ins	167–220	Genus Mycobacterium
Precorrin-4 C(11)-methyltransferase	WP_078061976	Supplementary Figure 5	1aa ins	159–206
NAD(P)H-quinone dehydrogenase	WP_062879231	Supplementary Figure 6	3aa ins	9–56
Orotidine 5′-phosphate decarboxylase	WP_062879058	Supplementary Figure 7	1aa del	39–71
Deoxyribonuclease IV	WP_031666830	Supplementary Figure 8	4aa del	109–149
Serine hydrolase	WP_062879883	Supplementary Figure 9	3aa ins	122–166
Peptidase C69a,b	WP_070410295	Supplementary Figure 10	1aa del	279–327
SGNH/GDSL hydrolase family proteina	WP_031743956	Supplementary Figure 11	1aa del	95–143
Succinate dehydrogenasea	WP_062880084	Supplementary Figure 12	1aa ins	27–56
N-dimethylarginine dimethylaminohydrolasea,b	SGA93253	Supplementary Figure 13	1aa del	109–153
Nif3-like dinuclear metal center hexameric protein	WP_066808468	Figure 4 Supplementary Figure 14	2aa del	31–75	Genus Mycobacterium except the “Abscessus-Chelonae” Clade
Phosphoribosylamine-glycine ligase	CKM81105	Supplementary Figure 15	5 aa ins	106–161
D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase	WP_083039002	Supplementary Figure 16	1aa del	418–449
Heat-inducible transcriptional repressor HrcA	WP_031668340	Supplementary Figure 17	2aa ins	193–228

^aOnly in comparison to other Corynebacteriales.

^bHomologues of Hoyosella species were absent in BLASTp searches.