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. 2018 Feb 14;12:8. doi: 10.3389/fnana.2018.00008

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Copyright © 2018 Tatsumi, Isonishi, Yamasaki, Kawabe, Morita-Takemura, Nakahara, Terada, Shinjo, Okuda, Tanaka and Wanaka.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Olig2-AS expressed low level of GFAP in the LGP and the SN. Olig2-AS co-expressed mature astrocytic marker SOX9 (arrow, A2), s100β (arrows, B2,C2) and GS (arrow, D2) in the LGP (A,B) and the SN (C,D). GFAP immunoreactive structures (arrowheads, A3–D3) were barely detected in the Olig2-AS territories (bordered with white dotted lines). Note that mature astrocyte markers (SOX9, s100β, and GS, arrowheads in A2–D2) were co-expressed with GFAP immunoreactivities (arrowheads in A4–D4). Scale bar (D5, for all panels): 20 μm.