Table 1.
List of primary antibodies used.
| Primary antibodies | Source | Identifier | Dilution |
|---|---|---|---|
| FOR IMMUNOFLUORESCENT STAINING | |||
| Rabbit anti-GFAP | Dako | Z0334 | 1:2,000 |
| Mouse anti-GFAP | EMD millipore | MAB360 | 1:500 |
| Rabbit anti-S100β | Abcam, Japan | AB41548 | 1:5,000 |
| Rabbit anti-3-PGDH | Frontiers Institute | Af303 | 1:6,000 |
| Mouse anti-GS | EMD millipore | MAB302 | 1:2,000 |
| Goat anti-SOX9 | R&D systems | #P48436 | 1:1,000 |
| Rabbit anti-GLAST | Cell signaling | #5684 | 1:1,000 |
| Rabbit anti-GLT-1 | Cell signaling | #3838 | 1:1,000 |
| Rabbit anti-GAT-3 | EMD millipore | AB1574 | 1:200 |
| Guinea pig anti-GAT-3 | Synaptic systems | Cat. No. 274301 | 1:2,000 |
| Rabbit anti-VGAT | Synaptic systems | Cat. No. 131013 | 1:200 |
| Guinea pig anti-VGIuT1 | Miyazaki et al., 2003 | RRID: AB_2571618 | 1 μg/ml |
| Guinea pig anti-VGluT2 | Miyazaki et al., 2003 | RRID: AB_2571621 | 1 μg/ml |
| Rabbit anti-GFP | Takasaki et al., 2010 | RRID: AB_2571573 | 3 μg/ml |
| FOR IMMUNOELECTRON MICROSCOPY | |||
| Rat anti-GFP | Nacalai tesque | GF090R | 1:500 |
| Rabbit anti-GFP | Takasaki et al., 2010 | RRID: AB_2571573 | 1 μg/ml |
| Rabbit anti-VGAT | Synaptic systems | Cat. No.l31013 | 1:3,000 |
| Guinea pig anti-VGluTl | Miyazaki et al., 2003 | RRID: AB_2571618 | 1 μg/ml |
| Guinea pig anti-VGluT2 | Miyazaki et al., 2003 | RRID: AB_2571621 | 1 μg/ml |
| Guinea pig anti-VIAAT | Miyazaki et al., 2003 | RRID: AB_2571624 | 1 μg/ml |