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. Author manuscript; available in PMC: 2018 Dec 28.
Published in final edited form as: J Control Release. 2017 Oct 13;268:49–56. doi: 10.1016/j.jconrel.2017.10.006

Figure 4. Homing of NWs coated with RGD peptides to non-responder tumors.

Figure 4

(A) Tumor-bearing mice were treated with the nanosystem for 3 weeks, and at the end of the treatment, FAM-labeled peptide-NWs (green) were intravenously injected (5mg iron/kg) and allowed to circulate for 5 hours. The mice were further injected with 100μg of DyLight 488 labeled Lycopersicon esculentum lectin, (green). After 5 minutes, the mice were perfused with PBS through the left ventricle of the heart, followed by 4% formaldehyde in PBS. Tumors and organs were collected and processed for fluorescence microscopy. (B) Quantification of NW accumulation in tumors. Slides were scanned using Scanscope. NW fluorescence in 10 random areas of each tumor section (n = 3/group) was quantified using ImageJ software. (****P < 0.0001, paired t test, n = 3 mice per group. Error bars = mean ±SD).