Table 1.
Inhibition and binding parameters for the various small-molecules.
| S. No | Compound | IC50 (μM) | Kiapp (μM)2 | ΔTm(°C)3 |
|---|---|---|---|---|
| 1 | SITS | 32.3 ± 1.8 | 32.3 | NS |
| 2 | flavonic acid | 36.7 ± 3.1 | 36.7 | NS |
| 3 | DNDS1 | 45.3 ± 10.1 | 45.3 | 1.9 |
| 4 | DIDS | 51.0 ± 1.7 | 51.0 | NS |
| 5 | oxyresveratrol | 81.8 ± 1.8 | 81.8 | 1.9 |
| 6 | resveratrol | 95.7 ± 3.0 | 95.7 | 8.3 |
| 7 | ononetin | 83.4 ± 1.1 | 83.4 | 3.2 |
1
dinitro disulfonic acid;
2
calculated theoretically with the assumption that it is non-competitive inhibition, i.e. Ki=IC50 when S=Km or S≫Km or S ≪ Km;
3
all the thermal shifts were done at a single concentration of 0.5 mM small molecule
NS, no signal possibly due to interference with the extrinsic fluorophore dye.