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. Author manuscript; available in PMC: 2018 Jul 21.
Published in final edited form as: ACS Chem Biol. 2017 May 31;12(7):1848–1857. doi: 10.1021/acschembio.7b00175

Table 2.

Inhibition parameters and mechanism of the small molecule ononetin for EcDHFR at different substrate and cofactor concentrations

Inhibitor Lower substrate* Higher substrate
ononetin Substrate Inhibition Mechanism αKi (μM) Ki (μM)#
H2F Uncompetitive 111.2 ± 12.37 22.15 ± 10.94
NADPH Uncompetitive 113.2 ± 13.1 72.69 ± 22.18
resveratrol H2F Linear Mixed-type 88.30 ± 6.02 31.48 ± 8.85
SITS H2F Non-competitive 39.63 ± 3.01 35.86 ± 11.23
flavonic acid H2F Non-competitive 76.25 ± 6.82 18.32 ± 9.68
*

The reported values are for the global non-linear fits.

#

values from fit to equation for substrate-inhibition at highest inhibitor concentration.

Note that the values reported here are not from global non-linear fits and differ from one inhibitor to another depending on the highest concentration employed for the respective inhibitor (Fig S5).