Figure 3. Expression of miR-205 in lung squamous cell carcinoma (SCC) and adenocarcinoma (AC) resectates.
Reverse transcription (RT)-PCR was used to quantify miR-205 relative to U6B and miR-21 (Lebanony score), or only U6B (miR-205 ΔCqU6B) in 5 ng RNA for 49 AC and 28 SCC cases. For each type of measurement, panel A shows dot-plots of individual values along with histology-specific group means and standard deviations; panel B shows receiver operating characteristic curves, along the area under curve (AUC) with 95% confidence interval, for the miR-205 measurements to discriminate SCC from AC; and, panel C shows the measurement values in terms of molarity along with linear regression lines and their slopes (m) and Y intercepts (b) determined by the least squares fitting technique. Molarity equivalent of the Lebanony score could be determined for only 56 of the total 77 cases.