Skip to main content
. 2018 Feb 7;14(2):e1007058. doi: 10.1371/journal.pgen.1007058

Fig 4. Gli3 deficiency affects taste cell differentiation and expression of Shh target genes.

Fig 4

(A-D) Indirect immunofluorescence confocal microscopy of taste organoids cultured from individual FACS-sorted Lgr5-GFP cells isolated from CV papillae of double-knockin mice treated with tamoxifen (Gli3CKO; C, D) or without (Gli3WT;A, B) and stained with antibodies against T1R3 (A, C) or GNAT3 (B, D). Scale bars, 100 μm. (E) The number of T1R3+ (n = 76, t = 3.05, p<0.01) but not GNAT3+ (n = 84, t = 0.61, p>0.05) cells increased in Gli3CKO organoids. (F) In Gli3CKO organoids vs. those from Gli3WT the expression of Tas1r3 mRNA (t = 8.87, p<0.001) increased dramatically, while that of Gna14(t =, p<0.01) and Gnat3 (t = 4.37, p<0.05) showed a modest increase, but that of Trpm5 (t = 1.17, p>0.05) remained unchanged. Data are means + SEM. *p<0.05, **p<0.01, ***p<0.001.