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. 2017 Nov 25;98(1):15–27. doi: 10.1093/biolre/iox161

Figure 5.

Figure 5.

The EGR2 transcription factor is regulated by PLZF in HESCs. (A) Snapshot of the UCSC Genome Browser depicting the PLZF ChIP-seq binding peak on the human EGR2 gene promoter. (B) Direct binding of PLZF at +124 and –452 base pairs from the transcriptional start site (TSS) in the EGR2 promoter was confirmed by ChIP-qPCR. (C) Sequence of PLZF binding interval on the EGR2 promoter with putative PLZF binding motif highlighted in red; primer sequences for +124 and –452 base pair regions used for ChIP-qPCR are highlighted in green and blue respectively. (D) Quantitative real-time PCR confirms that EGR2 transcript levels are markedly reduced with EPC treatment and that this reduction is significantly attenuated with PLZF knockdown. (E) Quantitative real-time PCR confirms that EGR2 transcript levels are significantly reduced by EPC treatment. Of the three EPC components, MPA significantly downregulates EGR2 transcript levels. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.