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. 2017 Mar 1;96(3):531–541. doi: 10.1095/biolreprod.116.145367

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© The Authors 2017. Published by Oxford University Press on behalf of Society for the Study of Reproduction.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please e-mail: journals.permissions@oup.com

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Figure 1. — Assessment of total cell numbers in bovine day 8 postfertilization blastocysts. Cumulative frequency distribution of the number of cells in blastocysts derived from the control (red line) and PGE2-treated MIV/FIV (0.1, 1. 10 μM PGE2 all together, blue line) groups (P < 0.05) (A). Distribution of day 8 postfertilization blastocysts derived from IVM/IVF cultures supplemented with increasing doses of PGE2, 243 cells being the maximum blastomere number counted in control embryos (P < 0.05) (B).