Table II.
Alignment of BCL2/IGH translocation sequences from matched samples of follicular lymphoma (FL) tumor and blood
| Case no. |
DNA source |
Tumor histology (ICD-O-3) |
Interval from blood draw to diagnosis (yr) |
Trans- location frequency per 100,000 cells |
BCL2-MBR break- point* |
Other BCL2 breakpoint |
Nontemplated N nucleotides† | N length (bp) |
JH gene |
IGH break- point‡ |
PCR fragment length (bp) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MBR1 | tumor | FL, grade 1 (M-9695) | 0.4 | 3056 | TGGGGACTAC | 10 | 6 | 2967 | 134 | ||
| blood | 16,600 | 3056 | TGGGGACTAC | 10 | 6 | 2967 | 134 | ||||
| MBR2 | tumor | FL, NOS (M-9690) | 0.7 | 3049 | TGAGTT | 6 | 4 | 1904 | 138 | ||
| blood | 0.9 | 3049 | TGAGTT | 6 | 4 | 1904 | 138 | ||||
| MBR3 | tumor | FL, NOS (M-9690) | 1.8 | 3114 | CTATTTCGGTT | 11 | 6 | 2962 | 199 | ||
| blood | 0.2 | 3114 | CTATTTCGGTT | 11 | 6 | 2962 | 199 | ||||
| MBR4 | tumor | FL, grade 3 (M-9698) | 3.0 | 3136 | AGATTCCGT | 9 | 5 | 2370 | 205 | ||
| blood | 8.4 | 3136 | AGATTCCGT | 9 | 5 | 2370 | 205 | ||||
| non-MBR1 | tumor | FL, grade 2 (M-9691) | 3.2 | 16250‖ (icr) | GCCGACGGGGGGGGT | 15 | 6 | 2954 | 222 | ||
| blood | 5.4 | 16250‖ (icr) | GCCGACGGGGGGGGT | 15 | 6 | 2954 | 222 | ||||
| MBR5 | tumor | FL, NOS (M-9690) | 4.5 | 3110 | AGTATCACGTATTATCCCGGGGCGGTTTAA-D-GAAA | 34 | 6 | 2949 | 246 | ||
| blood | 10,600 | 3110 | AGTATCACGTATTATCCCGGGGCGGTTTAA-D-GAAA | 34 | 6 | 2949 | 246 | ||||
| non-MBR2 | tumor | FL, NOS (M-9690) | 5.3 | 16248‖ (icr) | GAATCCCTTAAT | 12 | 6 | 2948 | 195 | ||
| blood | positive§ | 16248‖ (icr) | GAATCCCTTAAT | 12 | 6 | 2948 | 195 | ||||
| non-MBR3 | tumor | FL, grade 2 (M-9691) | 5.8 | 2988* | GCGAAGAAACACGAAACAGATCGTCGACCCCTCATCAGATAA | 42 | 4 | 1920 | 360 | ||
| blood | 1.4¶ | ||||||||||
| MBR6 | tumor | FL, grade 3 (M-9698) | 6.7 | 3113 | ACCCTATGGGTCTCCAT | 17 | 6 | 2965 | 200 | ||
| blood | 0.2 | 3113 | ACCCTATGGGTCTCCAT | 17 | 6 | 2965 | 200 | ||||
| MBR7 | tumor | FL, grade 3 (M-9698) | 7.1 | 3053 | CCTAAGTGGGCTATTTTTTTTGTCT | 25 | 6 | 2947 | 166 | ||
| blood | nonde-tectable | ||||||||||
| MBR8 | tumor | FL, NOS (M-9690) | 7.8 | 3158 | TCTGTC | 6 | 4 | 1922 | 227 | ||
| blood | 1.0 | 3158 | TCTGTC | 6 | 4 | 1922 | 227 | ||||
| MBR9 | tumor | FL, NOS (M-9690) | 8.2 | 3114 | 0 | 5 | 2366 | 176 | |||
| blood | positive§ | 3110 | GCCAC | 5 | 5 | 2371 | 174 |
*
BCL2 sequence based on GenBank accession number M14745.
†
D - indicates additional insertion of diversity gene IGHD3–3.
‡
IGH sequence based on GenBank accession number J00256.
§
Sample not quantitated due to assay failure.
‖
BCL2 sequence based on GenBank accession number AF325194.
¶
Sequence not determined due to assay failure.