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. Author manuscript; available in PMC: 2018 Feb 20.
Published in final edited form as: Mucosal Immunol. 2016 Jan 6;9(5):1340–1352. doi: 10.1038/mi.2015.133

Figure 6. α–CD205-p24 vaccine delivered i.n. induces long term memory in effector sites of the GI tract.

Figure 6

C57Bl/6 mice were immunized in a prime-boost regimen with polyICLC plus either α–CD205-p24 fusion mAb (5µg) or p24 (15µg, the highest dose of untargeted p24 vaccine was chosen). Mice were sacrificed 25 weeks post boost and lymphocytes were isolated from the spleen, lung and the effector sites (SILP and CLP) of the GI tract. IFN-γ+CD4+ T cells were examined by flow cytometry.

(A, B) FACS plots from a representative experiment showing antigen specific IFN-γ+CD4+ T cells in the spleen, lung, SILP and CLP. (C) Mean data from three independent experiments is shown (5 mice per group). Background (p17) subtracted p24 data are shown. Statistical comparisons between α-CD205-p24 fusion mAb and p24 are shown. Error bars show mean ± SD. *p<0.05, **p<0.01, ***p<0.001