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. Author manuscript; available in PMC: 2018 Feb 20.
Published in final edited form as: Mucosal Immunol. 2016 Jan 6;9(5):1340–1352. doi: 10.1038/mi.2015.133

Figure 7. Heterologous α–CD205-p24 prime, NYVAC gag/pol/nef boost immunization, generates p24-specific CD8+ T cells in the systemic and mucosal compartments.

Figure 7

F1 hybrid (cross between BALB/c females and C57Bl/6 males; heterozygous at all loci) mice were immunized with α–CD205-p24 fusion mAb (5µg), poly ICLC (50µg) and α-CD40 (25µg). After 4 weeks, the mice were administered 107 PFU NYVAC gag/pol/nef delivered i.n. After 2 weeks of boost, IFN-γ secretion was evaluated in CD8+ T cells.

(A) Shows the schema of immunization.

(B) FACS plots from a representative experiment, illustrating the induction of IFN-γ+CD8+ cells in the lung, vaginal tract, blood and spleen.

(C) Mean data from three independent experiments (5 mice per group) summarizing IFN-γ+CD8+ T cells in the lung, Blood, Spleen and vagina. Statistical comparisons to p17 (control) are shown. Error bars show mean ± SD. *=p<0.05, ***=p<0.001