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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Neurobiol Aging. 2017 Jun 17;58:1–13. doi: 10.1016/j.neurobiolaging.2017.06.003

Figure 6. GSK-3β activity is dysregulated concomitantly with peripheral insulin resistance in Tg2576 and 3xTg-AD mice.

Figure 6

(A) Representative western blots of protein extracted from both 5- and 9-month-old Tg2576 and WT mice. Blots were probed with the indicated antibodies. (B) GSK-3β levels were unaltered at 5-month-old and 9-month-old in Tg2576 mice compared to WT mice. (C) The excitatory phosphorylation site of GSK-3β at Tyr216 was enhanced in 9-month-old Tg2576 mice compared to WT mice (t(10) = 5.390, p < 0.01). (D) Representative western blots of protein extracted from both 10- and 16-month-old 3xTg-AD and WT mice. (E) No significant differences between 10-month-old WT and 3xTg-AD mice and between 16-month-old WT and 3xTg-AD mice for GSK-3β. (F) No significant differences for pGSK-3β at Tyr216 were detected between 10-month-old WT and 3xTg-AD mice. 16-month-old 3xTg-AD mice show significantly elevated levels of pGSK-3β at Tyr216 compared to WT mice (t(10) = 5.125, p < 0.05). Quantitative analyses of the blots obtained by normalizing the quantity of a specific protein with its loading control. Error bars represent mean ± SEM. *p<0.05, **p<0.01, ***p<0.001. n = 6/genotype/age.