Skip to main content
. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Transl Res. 2017 Aug 15;188:10–26. doi: 10.1016/j.trsl.2017.08.002

Fig. 8. Effect of Nano in altering AMPKα signaling in C2C12 differentiated myotubes.

Fig. 8

Micrographs (10X) of un-differentiated myoblasts (A) and differentiated myotubes at Day 7 (B) are shown. Myotubes were treated with native SOD or Nano for 6 h. Cells were rinsed and incubated in fresh growth media overnight. Cell lysates were analyzed for SOD1 uptake and markers of AMPK signaling. Representative bands were shown (C). Band intensities were quantified and normalized to β-actin (D–H). Values are expressed as mean ± SEM of 3 independent experiments. P values were determined using the 1-way ANOVA followed by Tukey’s multiple comparison test. Cont, Control, Nat, Native, and Nn, nanoSOD.