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. 2017 Dec 22;41(3):1365–1376. doi: 10.3892/ijmm.2017.3346

Figure 5.

Figure 5

Effects of p53-knockdown on the protein and mRNA expression levels of IFI16, AMPK and p-AMPK in HAMSCs (passage 3). HASMCs were untreated, or were transfected with control siRNA or p53 siRNA. (A) A total of 48 h post-transfection, immunofluorescence analysis was performed to detect endogenous p53 expression. (B) Histogram represents the ratio of p53 fluorescence intensity from six random fields. (C) A total of 48 h post-transfection, cells were lysed and examined by western blotting to detect p53, IFI16, AMPK and p-AMPK protein expression. (D) Histogram represents the fold change in p53, IFI16, AMPK and p-AMPK protein expression normalized to GAPDH relative to the untreated group. (E) A total of 24 h post-transfection, the mRNA expression levels of p53, IFI16 and AMPK were measured by reverse transcription-quantitative polymerase chain reaction. Histogram represents the fold change in p53, IFI16 and AMPK mRNA expression normalized to GAPDH relative to the untreated group. All data are presented as the means ± standard deviation from three independent experiments. **P<0.01 and ***P<0.001 vs. the untreated group. AMPK, 5′ adenosine monophosphate-activated protein kinase; Ctr, control; HASMCs, human aortic smooth muscle cells; IFI16, interferon-inducible protein 16; p-, phosphorylated; siRNA, small interfering RNA; Un, untreated.