Table II.
Gene | Primer sequences (5′→3′) | Product length (bp) |
---|---|---|
CREB1 | F: GGAGTGCCAAGGATTGAAGAAGA | 333 |
R: TGCTGTGCGAATCTGGTATGTT | ||
wtBRAF | F: GGCAGAGTGCCTCAAAAAGAA | 134 |
R: AACCAGCCCGATTCAAGGA | ||
GAPDH | F: GCACCGTCAAGGCTGAGAAC | 138 |
R: TGGTGAAGACGCCAGTGGA |
CREB1, cAMP responsive element binding protein 1; wtBRAF, wild-type B-Raf proto-oncogene, serine/threonine kinase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; F, forward; R, reverse.