Skip to main content
. 2018 Jan 9;41(3):1595–1607. doi: 10.3892/ijmm.2018.3373

Figure 1.

Figure 1

Osteogenesis is downregulated in the mandibles of diabetic rats and high-glucose-treated rBMSCs. (A) RT-qPCR analysis of the mRNA expression of ALP, OC, Runx2, Osterix, Smad1 and Col1 in the mandibles of high-fat diet- and STZ-induced type 2 diabetic, as well as in normal rats. (B) Histochemical detection of ALP of osteogenically-induced rBMSCs in high-glucose or normal medium on day 7 of culture. (C) Protein expression of Runx2 in rBMSCs osteogenically induced for 1, 4, 7, 10, 14 and 21 days was detected by western blot analysis. (D–G) RT-qPCR analysis of mRNA expression of Runx2, ALP, Osterix and OC in rBMSCs osteogenically induced in high-glucose and low-glucose medium for 0, 3, 6, 9, 12, 15, 18 and 21 days. Values are expressed as the mean ± standard deviation (n=3). *P<0.05, **P<0.01, ***P<0.001. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; L, low-glucose medium; H, high-glucose medium; rBMSCs, rat bone marrow mesenchymal stem cells; ALP, alkaline phosphatase; Runx2, runt-related transcription factor 2; Col1, collagen type 1 α 1; OC, osteocalcin.