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. 2018 Jan 10;41(3):1463–1468. doi: 10.3892/ijmm.2018.3375

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Copyright: © Wan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Generation of human neuronal cells by small molecules. (A) Scheme of induction procedure. (B) The cells display bipolar neuronal morphologies after 7 days introduction and 7 days maturation. Scale bars, 100 μm. (C) The chemicals induced-cells display express Tuj1 (green) and Tuj1⁺/Map2⁺. Scale bars, 75 mm. (D) The percentage of induced Tuj1⁺ and Tuj1⁺/Map2⁺. Neuronal cells in total cells at the time of quantification (means ± SD, n=10 random selected 50 fields from triplicate samples). V, VPA; C, CHIR99021; R, Repsox; F, forskolin; S, SP600625; Y, Y-27632; H, DMH1; S, SP600125; D, day; Tuj1, tubulin β 3 class III; Map2, microtubule-associated protein 2.