Table II.

Primers for reverse transcription quantitative polymerase chain reaction.

Gene	Forward	Reverse
Ascl1	5′-CAAGAGAGCGCAGCCTTAG-3′	5′-GCAAAAGTCAGTGCTGAACG-3′
Brn2	5′-AATAAGGCAAAAGGAAAGCAACT-3′	5′-CAAAACACATCATTACACCTGCT-3′
Myt1l	5′-CAATGGAAAGGGATTTTAAGCA-3′	5′-TTTGAGATTATGTACCAACGTTAGATG-3′
Ngn2	5′-TCAGACATGGACTATTGGCAG-3′	5′-GGGACAGGAAAGGGAACC-3′
NeuroD1	5′-GTTATTGTGTTGCCTTAGCACTTC-3′	5′-AGTGAAATGAATTGCTCAAATTGT-3′
Foxa2	5′-AGCAGAGCCCCAACAAGATG-3′	5′-TCTGCCGGTAGAAGGGGAAGA-3′
Sox2	5′-CAAGATGCACAACTCGGAGA-3′	5′-CGGGGCCGGTATTTATAATC-3′
Foxg1	5′-AGAAGAACGGCAAGTACGAGA-3′	5′-TGTTGAGGGACAGATTGTGGC-3′
Pax6	5′-GGCAACCTACGCAAGATGGC-3′	5′-TGAGGGCTGTGTCTGTTCGG-3′
Thy1	5′-ATCGCTCTCCTGCTAACAGTC-3′	5′-CTCGTACTGGATGGGTGAACT-3′
Ctgf	5′-CATCTCCACCCGGGTTACCAA-3′	5′-AGTACGGATGCACTTTTTGC-3′
Col1a1	5′-GAGGGCCAAGACGAAGACATC-3′	5′-CAGATCACGTCATCGCACAAC-3′
HPRT	5′-CCTGGCGTCGTGATTAGTGAT-3′	5′-AGACGTTCAGTCCTGTCCATAA-3′

The primers were mostly referenced from a previous study (8). Ascl1, achaete-scute family bHLH transcription factor 1; Brn2, POU class 3 homeobox 2; Myt1l, myelin transcription factor 1 like; Ngn2, neurogenin 2; NeuroD1, neuronal differentiation 1; Foxa2, forkhead box A2; Sox2, SRY-box 2; Foxg1, forkhead box G1; Pax6, paired box 6; Thy1, Thy-1 cell surface antigen; Ctgf, connective tissue growth factor; Col1a1, collagen type I α 1 chain; HPRT, hypoxanthine phosphoribosyltransferase.