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. 2017 Dec 22;41(3):1560–1572. doi: 10.3892/ijmm.2017.3341

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Ficz maintains the integrity and sufficient quantity of tight junctions (ZO-1 and occludin) following murine I/R. (A) Expression and distribution of ZO-1 and occludin in three groups (sham, I/R and I/R + Ficz) were detected by immunohistochemistry staining (original magnification, ×400; scale bar, 25 µm; n=3). (B) Western blotting of ZO-1, occludin and GAPDH protein levels. (C) Relative expression of proteins in (B), GAPDH was used to verify equivalent loading. Data are presented as the mean ± standard deviation. ^*P<0.05, ^**P<0.01, ^***P<0.001 vs. I/R group; n=3. I/R, ischemia/reperfusion; Ficz, 6-formylindolo(3,2-b)carbazole; ZO-1, tight junction protein 1.