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. 2017 Dec 22;41(3):1560–1572. doi: 10.3892/ijmm.2017.3341

Figure 6.

Figure 6

Ficz activates AhR-Notch1 signaling in Caco-2 cells under normoxic and hypoxic conditions. (A) Immunofluorescence staining was performed to evaluate the expression of AhR (green) and NICD1 (red) in four groups (Nx, Nx + Ficz, Hx and Hx + Ficz). Nuclei were counterstained with DAPI (blue; original magnification, ×1,200; scale bar, 25 µm; n=3). (B) Western blotting was used to measure the protein levels of AhR, CYP1A1, NICD1, HES-1 and GAPDH. (C) Relative protein expressions determined by densitometry, GAPDH was used to verify equivalent loading. Data are presented as the mean ± standard deviation. *P<0.05, **P<0.01, ***P<0.001 vs. Nx group; ##P<0.01, ###P<0.001 vs. Hx group; n=3. AhR, aryl hydrocarbon receptor; NICD1, Notch1 intracellular domain; Nx, normoxia; Ficz, 6-formylindolo(3,2-b)carbazole; Hx, hypoxia; CYP1A1, cytochrome P450; HES-1, hes family bHLH transcription factor 1.