Table I.

Primers used for reverse transcription-quantitative polymerase chain reaction quantification of differentially expressed miRNAs and inflammation-associated mRNAs.

miRNA/mRNA	Primer sequence
rno-miR-132-3p
RT primer	5′-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCGACCAT-3
Forward	5′-GCGTAACAGTCTACAGCCAT-3′
Reverse	5′-CAGTGCGTGTCGTGGAGT-3′
rno-miR-182
RT primer	5′-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCGGTGTG-3′
Forward	5′-GGTTTGGCAATGGTAGAACTCA-3′
Reverse	5′-CAGTGCGTGTCGTGGAGT-3′
rno-miR-208b-3p
RT primer	5′-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACACCTTTT-3′
Forward	5′-GGCCGGGATAAGACGAACAA-3′
Reverse	5′-CAGTGCGTGTCGTGGAGT-3′
rno-miR-212-3p
RT primer	5′-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTGGCCGT-3′
Forward	5′-TAACAGTCTCCAGUCACGGCCA-3′
Reverse	5′-CAGTGCGTGTCGTGGAGT-3′
rno-miR-214-3p
RT primer	5′-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCTGCCTG-3′
Forward	5′-CGACAGCAGGCACAGACA-3′
Reverse	5′-CAGTGCGTGTCGTGGAGT-3′
rno-miR-218a-5p
RT primer	5′-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACACATGGT-3′
Forward	5′-GCGGTTGTGCTTGATCTAAC-3′
Reverse	5′-CAGTGCGTGTCGTGGAGT-3′
rno-miR-221-3p
RT primer	5′-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACGAAACCC-3′
Forward	5′-GGAGCTACATTGTCTGCTGG-3′
Reverse	5′-CAGTGCGTGTCGTGGAGT-3′
rno-miR-222-3p
RT primer	5′-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACACCCAGT-3′
Forward	5′-AGCUTACATCTGGCTACTGGGT-3′
Reverse	5′-CAGTGCGTGTCGTGGAGT-3′
U6
RT primer	5′-AACGCTTCACGAATTTGCGT-3′
Forward	5′-CTCGCTTCGGCAGCACA-3′
Reverse	5′-AACGCTTCACGAATTTGCGT-3′
TNF-α
Forward	5′-ATGGGCTCCCTCTCATCAGT-3′
Reverse	5′-GCTTGGTGGTTTGCTACGAC-3′
ICAM-1
Forward	5′-ACCTGGACAAGAAGGACTGC-3′
Reverse	5′-GGTCAGATTAGGGGCTGGAT-3′
VCAM-1
Forward	5′-TGGGAAACTGGAAAGAGGAA-3′
Reverse	5′-CATCAGGAGCCAAACACTTG-3′
β-actin
Forward	5′-GAAGTGTGACGTTGACATCCG-3′
Reverse	5′-TGCTGATCCACATCTGCTGGA-3′

ICAM-1, intercellular cell adhesion molecule-1; miR/miRNA, microRNA; RT, reverse transcription; VCAM-1, vascular cell adhesion molecule-1.