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. 2017 Dec 15;41(3):1331–1338. doi: 10.3892/ijmm.2017.3323

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Copyright: © Sun et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of the chloride channel blocker 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) and the chloride channel activator lubiprostone (LUBI) on human conjunctival fibroblast (HConF) cell migration, assessed in vitro by performing scratch-wound and Transwell migration assays. The migratory ability of HConFs was determined by measuring the width of the scratch wound and the number of cells migrating to the lower chamber after 12 h. (A) Representative images of the scratch-wound assay results and quantification of the inhibitory effects of NPPB on HConF migration. (B) Representative images of Transwell migration assay results and quantification of the inhibitory effects of NPPB on HConF migration (^**P<0.01 vs. control). DMSO, dimethyl sulfoxide.