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. 2017 Dec 19;41(3):1323–1330. doi: 10.3892/ijmm.2017.3331

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Copyright: © Cui et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Metabotropic glutamate receptor 5 (mGluR5) antagonist attenuates the binding of Toll-like receptor 4 (TLR4) and glutamate receptor N-methyl-D-aspartate subunit 1 (GluN1) induced by lipopolysaccharide (LPS) in N9 and EOC 20 microglial cells. (A and B) N9 and (C and D) EOC 20 microglial cells were pretreated with 0.1 mM 3-[(2-methyl-1,3-thiazol-4-yl)ethynyl]pyridine (MTEP) for 30 min, and then treated with 1 µg/ml LPS for 1 h. Anti-TLR4 immunoprecipitates from (A) N9 or (C) EOC 20 microglial cells were blotted with anti-GluN1. Total cell lysates from (B) N9 or (D) EOC 20 microglial cells were blotted with anti-TLR4 (B and D, top panel), anti-GluN1 (B and D, middle panel), or anti-β-actin (B and D, bottom panel). Similar results were obtained from four independent experiments (n=4).