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NO donors SNP and GSNO can release NO into the medium of cultured glioma cells. The Griess method was used to quantify NO levels in the culture supernatant of U251 cells following (A) 1 and 2 mM SNP or (B) 250 and 500 µM GSNO treatment. **P<0.01 and ***P<0.001 compared with the untreated group (0). GSNO, S-nitrosoglutathione; NO, nitric oxide; SNP, sodium nitroprusside.
